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Exosome Diagnostics
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Bio-Techne corporation
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Cosmo Bio USA
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New England Biolabs
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NanoFCM Inc
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Image Search Results
Journal: International Journal of Nanomedicine
Article Title: Proteomic Landscape of Small Extracellular Vesicles Derived From Gastric Juice and Identified TFF2 as a Specific Biomarker
doi: 10.2147/IJN.S516605
Figure Lengend Snippet: Characteristics of gsEVs isolated by four methods. ( A ) The size of gsEVs were detected by NTA (Upper panel: statistical charts of particle size; Lower panel: representative images of particle size distribution). ( B ) The concentration of gsEVs particles (Upper panel: particle number per milliliter was detected by NTA; Lower panel: particle number per microgram of protein). ( C ) The morphological characteristics of gsEVs were detected by TEM. ( D ) The sEVs markers (Alix, CD63, CD9 and TSG101) and the non-sEVs marker (Calnexin) were detected by Western blotting. In the UC and PEG-UC methods, 20 μg of protein was loaded; in the UC-SEC and UF-SEC methods, 20 μL of each fraction was loaded. ( E ) The membrane markers of sEVs (CD9, CD63 and CD81) were detected by NanoFCM.
Article Snippet: In the UC and PEG-UC methods, 20 μg of protein was loaded; in the UC-SEC and UF-SEC methods, 20 μL of each fraction was loaded. ( E ) The membrane markers of
Techniques: Isolation, Concentration Assay, Marker, Western Blot, Membrane
Journal: International Journal of Nanomedicine
Article Title: Proteomic Landscape of Small Extracellular Vesicles Derived From Gastric Juice and Identified TFF2 as a Specific Biomarker
doi: 10.2147/IJN.S516605
Figure Lengend Snippet: TFF2 is specifically expressed in sEVs derived from gastric mucosal cells. ( A ) The particle size distribution of sEVs derived from gastric mucosal cells GES1-NC and GES1-TFF2-OE. ( B ) The morphological characteristics of cell culture medium-derived sEVs were observed by TEM. ( C ) The sEVs markers (Alix, CD63 and TSG101), the non-sEVs marker (Calnexin), and TFF2 were detected in Cell lysis, ultracentrifugation supernatant, and sEVs. ( D and E ) The expression levels of membrane markers of sEVs (CD9, CD63, and CD81) and TFF2 were detected by bead-bound flow cytometry in sEVs derived from GES1-NC and GES1-TFF2-OE cells.
Article Snippet: In the UC and PEG-UC methods, 20 μg of protein was loaded; in the UC-SEC and UF-SEC methods, 20 μL of each fraction was loaded. ( E ) The membrane markers of
Techniques: Derivative Assay, Cell Culture, Marker, Lysis, Expressing, Membrane, Flow Cytometry
Journal: International Journal of Nanomedicine
Article Title: Proteomic Landscape of Small Extracellular Vesicles Derived From Gastric Juice and Identified TFF2 as a Specific Biomarker
doi: 10.2147/IJN.S516605
Figure Lengend Snippet: TFF2 is specifically expressed in sEVs derived from GJ. ( A ) The particle size distribution of sEVs derived from GJ in mice and human. ( B ) The morphological characteristics of gsEVs derived from mice and humans were observed by TEM. ( C ) The sEVs markers, non-sEVs marker, and TFF2 were detected in GJ, ultracentrifugation supernatant, and gsEVs derived from mice. ( D ) The sEVs markers, non-sEVs marker, and TFF2 were detected in GJ, ultracentrifugation supernatant, and gsEVs derived from human. ( E and F ) The expression levels of membrane markers of sEVs (CD9, CD63 and CD81) and TFF2 was detected by bead-bound based flow cytometry in human gsEVs. ( G )The expression levels of sEV markers, including CD63, Syntenin-1, and TFF2, were analyzed in human gsEVs using immunogold labeling and TEM.
Article Snippet: In the UC and PEG-UC methods, 20 μg of protein was loaded; in the UC-SEC and UF-SEC methods, 20 μL of each fraction was loaded. ( E ) The membrane markers of
Techniques: Derivative Assay, Marker, Expressing, Membrane, Flow Cytometry, Labeling